Architects of the Genome: Using genomic technologies to elucidate the role of CTCF in neurodevelopmental disorders

Project Code

MRCNMH26Ex Flynn

Project Type

The project is primarily wet-lab based, with a dry-lab component in Years 2 and 3.

Research Theme

Neuroscience and Mental Health

Project Summary Download

Summary

Genetic variants in the CTCF gene, which encodes a protein that organizes DNA, cause neurodevelopmental disorders including intellectual disability and autism. However, the mechanisms responsible are not well understood. This project will use human stem cell models to investigate how CTCF variants disrupt DNA organization controls brain development. You will examine whether (i) binding of CTCF to DNA, (ii) chromatin structure and function and (iii) neural differentiation and brain organoid development are affected. You will also explore approaches to rescue the developmental defects. The student will gain valuable training in stem cell culture, genomics, and bioinformatic analyses of large datasets.

Lead Supervisor

Dr Sean Flynn

Lead Supervisor Email

S.M.Flynn@exeter.ac.uk

University Affiliation

Exeter